Product Description
ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase.
- Substrate Type: HRP(Horseradish Peroxidase)
- Description: Pierce 1-Step ABTS Solution
- Substrate Properties: Chemical Substrate
- Product Line: 1-Step
High Sensitivity for Peroxidase-based AssaysABTS Substrate Solution offers exceptional sensitivity for peroxidase assays, making it ideal for ELISA and other enzyme immunoassays. With its optimal formulation and high purity, researchers can trust this product to deliver consistent and reliable colorimetric data.
Convenient and Ready to UseThis substrate solution comes ready to use in liquid form; no additional reagents or mixing are needed. The single-solution format simplifies workflow, reduces preparation errors, and saves valuable laboratory time.
Safe and Stable PackagingPackaged in 100 mL, 500 mL, and 1 L bottles, ABTS Substrate Solution is non-hazardous and can be safely transported by ground or air. Its stability is assured for up to 12 months when stored at the recommended 2-8C, protected from light.
FAQ's of ABTS Substrate Solution:
Q: How should ABTS Substrate Solution be stored to maintain its stability?
A: Store ABTS Substrate Solution at 2-8C, protected from light, to ensure stability and maximize its shelf life of 12 months.
Q: What is the proper method for inactivating the ABTS reaction?
A: To stop the reaction, add sulfuric acid or hydrochloric acid as a stop solution to ensure safe and rapid inactivation.
Q: When should this substrate solution be used in ELISA protocols?
A: ABTS Substrate Solution should be added after the peroxidase-conjugated detection step and incubated until the appropriate color develops, with absorbance measured at approximately 415 nm.
Q: Where is ABTS Substrate Solution commonly applied?
A: ABTS is widely used in ELISA and peroxidase-based enzyme immunoassays within research laboratories for colorimetric detection.
Q: What are the main benefits of using ABTS Substrate Solution over other substrates?
A: ABTS Substrate Solution provides high sensitivity, clear colorimetric results, convenience as a ready-to-use reagent, and safety, as it is not classified as hazardous.
Q: Is ABTS Substrate Solution hazardous or poisonous to handle?
A: No, ABTS Substrate Solution is classified as non-hazardous and non-poisonous under recommended usage and is safe for standard laboratory handling and transport.